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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: DENEDDYLASE1 Protein Counters Automodification of Neddylating Enzymes to Maintain NEDD8 Protein Homeostasis in Arabidopsis
doi: 10.1074/jbc.M116.767103
Figure Lengend Snippet: AXR1 neddylation correlates with reduced RCE1∼NEDD8 thioester formation. A, anti-NEDD8 IB analysis of crude protein extracts (60 μg) after non-reducing or reducing SDS-PAGE. NEDD8 conjugates (−N8) and NEDD8 thioesters (∼N8) are labeled accordingly. B, anti-NEDD8 immunoblot analysis of crude protein extracts (60 μg) after non-reducing SDS-PAGE. Seedlings were grown for 7 days on growth medium with or without 10 μm MLN4924. The CDC2 IB serves as a loading control. C, signal intensity profile comparison of B for treated (light blue) and untreated wild type (WT) (dark blue) and untreated WT and den1-1 mutant (pink) samples generated with the MultiGauge analysis software.
Article Snippet: Immunoblot signal intensities were quantified with the
Techniques: SDS Page, Labeling, Western Blot, Mutagenesis, Generated, Software